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I have upgraded my OS to Windows 10 and SQL Server 2008 R2 Express to SQL Server 2012 Enterprise Edition.

Now I am getting error "The user instance login flag is not supported on this version of SQL Server. The connection will be closed." as I have search all the link and followed all the step but not getting rid of this error.

As I don't have any user instance =.... in my connection string.

I have gone through the following links as well

https://social.msdn.microsoft.com/Forums/en-US/8525a00a-d6ea-4630-94ef-fefbce198b62/the-user-instance-login-flag-is-not-supported-on-this-version-of-sql-server-the-connection-will-be?forum=sqlexpress
http://codeverge.com/asp.net.installation-setup/the-user-instance-login-flag-is-no/288955
http://answers.flyppdevportal.com/categories/sqlserver/sqlexpress.aspx?ID=8525a00a-d6ea-4630-94ef-fefbce198b62 The user instance login flag is not supported on this version of SQL Server. The connection will be closed

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Check your web.config file. I had faced similar issue but got it sorted after making changes in Connection String in web.config file